Omega-3 and omega-6 blood test: what the profile measures

BPR Clinic20 October 20265 min readLer em português

The Omega 3/Omega 6 Profile turns your fish intake into numbers: the omega-3 index, the omega-6 to omega-3 ratio and more. Here is what each marker means and when people tend to check it.

Tests in this article:Omega 3/Omega 6 Profile

You may eat salmon every week, or you may not have touched oily fish in months. Either way, it is hard to know what that means inside the body. An omega-3 blood test turns those habits into numbers. The Omega 3/Omega 6 Profile in the BPR catalogue looks at the balance of these fatty acids in your blood, which can be a useful check if you are changing your diet or taking a fish oil supplement.

Omega-3 and omega-6 in a nutshell

Omega-3 and omega-6 are families of polyunsaturated fats. The body cannot make enough of some of them, so they need to come from food. The long-chain omega-3s, EPA and DHA, are found mainly in oily fish. The NHS recommends at least 2 portions of fish a week, including 1 of oily fish such as salmon, sardines, mackerel, herring, trout or pilchards, and notes that fresh and canned tuna do not count as oily fish. It also explains that long-chain omega-3 is important during pregnancy and breastfeeding, because it helps the baby's nervous system develop.

Omega-6 fats are also part of an ordinary diet. Neither family is "good" or "bad" on its own. The interest lies in the balance and in how much omega-3 actually reaches the body's cells.

What the profile measures

LML lists four markers in the Omega 3/Omega 6 Profile:

Omega-3 index

The omega-3 index is the proportion of EPA and DHA among all the fatty acids in red blood cell membranes. It was proposed as a marker linked to heart health and has been studied in many populations (von Schacky, 2014). Because red blood cells are replaced over a period of months, the index reflects longer-term intake rather than your last meal.

Omega-6 to omega-3 ratio

This ratio compares the two families. LML notes that a better balance, with relatively more omega-3, is generally considered favourable and has been linked with lower inflammation and heart and brain health. It is worth knowing that researchers debate how useful the ratio is on its own. One critical review argues that it says less than the omega-3 index, because very different diets can produce the same ratio (Harris, 2018). That is why the profile reports both.

AA:EPA ratio

This compares arachidonic acid (AA), an omega-6 fat, with EPA, an omega-3 fat. It is another way of looking at the balance between the two families.

Omega trans fat index

This reflects the share of trans fats in the blood.

How omega-3 levels vary

A 2024 update that mapped omega-3 levels in blood across many countries found large differences between populations (Schuchardt et al., 2024). Within any population, individual results also vary a lot. A test is the only way to see where you sit, instead of assuming.

When does the test tend to make sense?

People commonly look at their omega-3 status when they:

  • eat little or no oily fish, for example on vegetarian or vegan diets;
  • take a fish or algae oil supplement and want to see whether it is changing anything;
  • are planning a pregnancy and want to review their diet with a professional;
  • train regularly; interest in the omega-3 index as a marker in sport is growing (Medoro et al., 2024).

As a general guide, people who track their omega-3 index often retest every 6 to 12 months, which gives enough time for changes in diet or supplements to show. Talk to a professional about whether and how often the test is useful for you, especially if you take blood-thinning medicines or other supplements.

What the result does and does not tell you

The profile shows the fatty acid balance in your blood. It does not, by itself, say anything certain about your heart, brain or any other part of the body. LML notes that the ratio is associated with various health conditions, and the research supports interest in these markers, but a result is one piece of information, not a verdict. A professional can put it in context with your diet, other tests such as cholesterol and your overall health.

Practical points

  • LML asks for this sample to reach the laboratory without delay, so a collection point or home phlebotomist may be more practical than a home kit.
  • Results are available about 2 weeks after the sample arrives at the laboratory, according to LML.
  • Write down any omega-3 supplement you take, including the dose, so the result can be read in context.

How the sample is collected

BPR does not collect samples. LML arranges all collections, either with a home kit, at an LML collection point (search by postcode) or with a phlebotomist who visits you at home. Anyone under 18 attending a collection point must be accompanied by an adult.

The Omega 3/Omega 6 Profile is in the BPR catalogue. If you have questions before ordering, write to [email protected], use the form on our website or message us in the app.

Frequently asked questions

Do I need to fast for the omega-3 test? LML does not list fasting for this profile. Because the omega-3 index reflects red blood cell membranes, it changes slowly and is not much affected by a single meal.

I take fish oil. How long before a retest shows a difference? Red blood cells are replaced over months, so changes tend to show gradually. Many people retest after 6 to 12 months; a professional can suggest the right timing.

Is a vegan diet compatible with good omega-3 levels? The NHS suggests that people on a vegan diet eat nuts and seeds rich in omega-3, such as walnuts, every day. A test can show how your current approach is working.

References

  1. Fish and shellfish - NHS. nhs.uk
  2. The vegan diet - NHS. nhs.uk
  3. von Schacky C. Nutrients. 2014;6(2):799-814. PMID 24566438
  4. Harris WS. Prostaglandins Leukot Essent Fatty Acids. 2018;132:34-40. PMID 29599053
  5. Schuchardt JP, et al. Prog Lipid Res. 2024;95:101286. PMID 38879135
  6. Medoro A, et al. J Funct Morphol Kinesiol. 2024;9(2). PMID 38804457

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This article is for general information and education only and is not a substitute for an individual assessment or treatment by a qualified healthcare professional. If you have significant, worsening or concerning symptoms, please seek advice from a suitably qualified clinician.