Blood tests for athletes: iron, vitamin D, recovery and energy

Blood tests for athletes: iron, vitamin D, recovery and energy

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BPR Clinic29 October 20268 min readLer em português

Which blood markers sports medicine teams look at, what each one means, and how to time tests through the season so the results are worth comparing.

Tests in this article:Sports Fitness Profile,Sports Hormone Profile,Iron Status Profile,Ferritin,Full Blood Count (with 5 part White Cell Differential),Vitamin D,Vitamin B12,Folate (Vitamin B9),Creatine Kinase,Thyroid Monitoring

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Your splits are slower than last month, the legs feel heavy on every warm-up and you are sleeping more but waking up flat. Is it the training block, the dark mornings, or something in your blood? Blood tests for athletes cannot answer that on their own, but used well they can take some of the guesswork out of it. This guide explains which markers sports medicine teams commonly look at, what each one means, and how to time your tests so the results are worth comparing.

Why blood tests for athletes are read differently

Training changes the blood. Endurance work expands plasma volume, so haemoglobin can read slightly low in a well-trained athlete without true anaemia. A hard session or a race raises muscle and inflammation markers for a few days. And people differ: a level that is normal for one athlete can be unusual for another.

A review of the blood markers used to manage training load concluded that the conditions of the sample, differences between individuals and each athlete's usual workload all move resting values, and that population reference ranges are of limited use on their own. Its advice is to compare each result with your own baseline, taken in the same conditions (Haller et al., 2023). That is the thread running through this article: one result is a snapshot, a series is a story.

Iron and ferritin: the most common gap

Iron carries oxygen in the blood and is part of the enzymes muscles use to make energy. Low iron is common in athletes, especially women, endurance athletes, vegetarians and anyone who trains at altitude, and it can exist before haemoglobin falls (Sim et al., 2019). Ferritin, the body's iron store, is the number most teams watch. Because inflammation and a hard session can push ferritin up, it is usually read with C-reactive protein (CRP) and the full blood count.

Rowers on the water in Bristol harbour

For women who train, periods matter too. A practical review for female athletes recommends looking at iron alongside the menstrual cycle pattern, training load and environment over time, rather than a single ferritin value (Pedlar et al., 2018).

Vitamin D through UK winters

Between October and early March the sun in the UK is not strong enough for the skin to make enough vitamin D, and government advice is that everyone should consider a daily supplement in autumn and winter (NHS). The UK's Scientific Advisory Committee on Nutrition sets a reference intake of 10 micrograms a day for everyone aged 4 and over, all year round, to protect bone and muscle health (SACN, 2016). Athletes who train indoors, cover up or have darker skin are more likely to run low, and late autumn or winter is a sensible time to check.

B12 and folate: diet first

Vitamin B12 and folate are needed to make red blood cells. B12 comes mainly from meat, fish, milk and eggs, so a vegan diet needs a reliable source, usually fortified foods or a supplement (Rogerson, 2017). Cutting out food groups to make weight raises the same question.

A sprinter in the starting position on a running track

Recovery and training load

Creatine kinase (CK) leaks from muscle into the blood after hard or unfamiliar exercise, especially eccentric work such as downhill running or heavy lifting. Urea can rise with endurance load, and CRP with a race or a block of hard sessions. None of these tells you on its own that you are overtrained. They only mean something against your own previous results, taken at the same time of day and at the same point in your training week (Haller et al., 2023).

Energy availability, thyroid and hormones

When food intake does not cover what training burns, the body starts saving energy. The International Olympic Committee (IOC) calls the result relative energy deficiency in sport (REDs), and it can affect hormones, bone, mood, immunity and performance in both women and men. The IOC's 2023 consensus lists blood signs that can go with low energy availability: a low free T3 (the active thyroid hormone), low testosterone in men, low oestradiol with missed or irregular periods in women, and raised total or low-density lipoprotein (LDL) cholesterol (Mountjoy et al., 2023).

These are clues, not a label. If your periods have stopped, you keep picking up stress injuries or your performance falls despite training well, see your GP or a sports doctor. Blood tests are one part of that assessment, alongside your history, diet and training.

Hydration markers, with care

Sodium and the kidney markers (urea and creatinine) are often part of an athlete panel. Sodium can go up with dehydration and down if you drink a lot of plain fluid, for example during long events. Creatinine depends on muscle mass and can read higher in muscular athletes, and recent hard training or high protein intake can raise urea. Read them with whoever reviews your results, not in isolation.

What each marker means

Watch: what each marker means

30 seconds · AI voice. They explain the words, not your result. Take your results to a doctor you trust, such as your GP.
  • Ferritin: stores iron. A low level may suggest iron deficiency, which can cause tiredness and weakness; a high level may point to excess iron. Inflammation and hard training can raise it, so it is read with CRP. Glossary
  • Haemoglobin: the protein in red blood cells that carries oxygen. A low level can point to anaemia; in trained athletes it can read slightly low because of extra plasma. Glossary
  • Transferrin saturation: how much iron is being carried in the blood. Low levels may mean the body is not getting or absorbing enough iron. Glossary
  • Vitamin D (25-hydroxyvitamin D): helps the body absorb calcium and keep bones and muscles strong. It comes from food, supplements and sunlight on the skin. Glossary
  • Vitamin B12 and folate: needed to make red blood cells; low B12 can bring anaemia, tiredness and nerve symptoms. Particularly relevant on a plant-based diet. B12 and folate in the glossary
  • CK: an enzyme from the muscles released after strenuous exercise. Hard training in the days before the sample can raise it, so note what you did when you share the result. Glossary
  • CRP: made by the liver in response to inflammation. A hard session or a race raises it for a short time. Glossary
  • Thyroid stimulating hormone (TSH), free T4 and free T3: TSH regulates the thyroid, and free T4 and free T3 are the thyroid hormones that set metabolism and energy. A low free T3 is one of the IOC's signs of low energy availability. TSH, free T4 and free T3
  • Testosterone and oestradiol: sex hormones in men and women. Testosterone changes through the day, so keep the same time of day for each test; the best time to give a sample is between 7am and 10am. Testosterone and oestradiol
  • Sodium, urea and creatinine: sodium helps balance the body's fluids; urea and creatinine are waste products the kidneys filter. Hydration, protein intake and muscle mass all move them. Sodium, urea and creatinine

Reference ranges vary; take your results to a doctor you trust, such as your GP.

When to test, and how to keep results comparable

  • Pre-season or before a new block: a baseline to compare everything else against.
  • Mid-season or in a heavy block: to see how you are carrying the load.
  • Late autumn or winter: for vitamin D.
  • After an injury, an illness or a change of diet: so your return starts from known numbers.

Keep the conditions the same each time: same time of day, a rest day or an easy day before the sample, normal food and fluid, and no race in the previous few days. Write down your training that week. Small changes between two tests are often within normal day to day variation, so look at the trend with your GP or sports doctor rather than a single number.

If you get chest pain, faint or feel your heart racing during exercise, stop and call 999. For urgent advice when you are not sure, call NHS 111.

Frequently asked questions

Should I stop training before a blood test? You do not need to stop, but avoid a hard or unfamiliar session in the 24 to 48 hours before, so muscle and inflammation markers reflect your usual state.

My ferritin is low but my haemoglobin is normal. Does that matter? Low iron stores can exist before haemoglobin falls. Take the result to a doctor you trust, such as your GP or a sports doctor; do not start iron on your own, because too much iron also causes problems.

How often should athletes test? Many teams check iron about twice a year and add a test mid-season or in winter. Your GP or sports doctor can suggest an interval that fits your sport and history.

Next steps

Our blood tests for athletes page sets out each marker with the research behind it. For your sport, see the pages for endurance, strength and gym and contact sports, the sport and training card in the check-up guide, and the sports fitness profile. Tests are processed by our partner laboratory, and you can share the results with your GP, sports doctor or therapist from the app. If you are planning to repeat tests through the season, our article on repeating blood tests over time explains how to make the series useful.

This content is for information and education only and does not replace an assessment or treatment by a qualified health professional. Always talk to your GP or a health professional for individual advice.

References

  1. Mountjoy M, Ackerman KE, Bailey DM, et al. Br J Sports Med. 2023;57(17):1073-1097. PMID 37752011
  2. Haller N, Behringer M, Reichel T, et al. Sports Med. 2023;53(7):1315-1333. PMID 37204619
  3. Sim M, Garvican-Lewis LA, Cox GR, et al. Eur J Appl Physiol. 2019;119(7):1463-1478. PMID 31055680
  4. Pedlar CR, Brugnara C, Bruinvels G, Burden R. Eur J Sport Sci. 2018;18(2):295-305. PMID 29280410
  5. Rogerson D. J Int Soc Sports Nutr. 2017;14:36. PMID 28924423
  6. SACN vitamin D and health report - GOV.UK. gov.uk
  7. Vitamin D - NHS. nhs.uk

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This article is for general information and education only and is not a substitute for an individual assessment or treatment by a qualified healthcare professional. If you have significant, worsening or concerning symptoms, please seek advice from a suitably qualified clinician.